Table 1.

Comparison of CYP gene expressions in HepG2, HepG2-empty vector, CYP-overexpressing HepG2 cells and primary human hepatocytes.

	Expression Value*									Expression Value in CYP-overexpressing cells vs human hepatocytes (fold change level**)
	HepG2		HepG2-empty vector		CYP-overexpressing HepG2			Human Hepatocytes
CYP Gene	Average	S.D.	Average	S.D.	Cell lines	Average	S.D.	Average	S.D.
CYP1A1	2.10	0.62	3.12	0.20	HepG2-CYP1A1	9230.86	570.06	3.15	0.37	+++
CYP1A2	N.D.		N.D.		HepG2-CYP1A2	6085.21	316.73	28.38	1.63	+++
CYP1B1	N.D.		N.D.		HepG2-CYP1B1	7260.78	301.46	28.91	1.42	+++
CYP2A6	0.34	0.07	0.36	0.17	HepG2-CYP2A6	14958.27	1494.98	1330.04	80.42	++
CYP2B6	34.56	0.76	32.29	0.80	HepG2-CYP2B6	6310.56	909.17	869.33	71.43	+
CYP2C8	N.D.		N.D.		HepG2-CYP2C8	3624.38	546.40	1122.95	44.64	+
CYP2C9	N.D.		N.D.		HepG2-CYP2C9	3848.98	196.82	1284.64	114.59	+
CYP2C18	0.04	0.00	0.04	0.00	HepG2-CYP2C18	7289.49	420.42	341.21	30.01	++
CYP2C19	N.D.		N.D.		HepG2-CYP2C19	4568.73	295.48	35.96	0.71	+++
CYP2D6	N.D.		N.D.		HepG2-CYP2D6	525.41	83.72	13.50	3.36	++
CYP2E1	0.04	0.01	0.04	0.00	HepG2-CYP2E1	2005.09	132.22	5223.96	348.51	−
CYP3A4	N.D.		N.D.		HepG2-CYP3A4	1849.10	237.69	838.40	7.13	+
CYP3A5	1.57	0.15	1.99	0.08	HepG2-CYP3A5	10034.07	853.00	872.15	83.79	++
CYP3A7	1.38	0.15	1.57	0.04	HepG2-CYP3A7	6466.39	211.30	11.60	1.48	+++

N.D., not detected.

^*Expression Value is calculated based on the assumption that the expression level of the housekeeping gene GAPDH is 10,000 copies (10, 000 is arbitrary). E = 2^−ΔCt * 10,000; ΔCt = Ct of test gene-Ct of GAPDH.

^**Fold change level was defined as +++, fold change > 100;++, fold change = 10–100; +, fold change = 1–10; −, fold change = 0–1.