Fig. 1.

Cyclophilin D disruption alters the inflammatory response during LPS‐induced sterile shock in mouse liver. (A) LPS‐induced expression of Tnfα, Ifnγ, Cxcl2, and Csf1 at 2 h (proinflammatory phase) and 24 h (immunosuppressed phase) were determined by qPCR. Expression values were normalized to β‐actin. Data are presented as mean ± SEM of FC values (n = 5) relative to the expression in the WT group. *P < 0.05, independent samples t‐test at both time points. (B) qPCR validation of Cd14, Tbk1, Tlr2, and Tank mRNAs levels in sequencing data during LPS‐induced endotoxemia in WT and CypD^−/− mice liver. Data are presented as mean ± SEM of FC values (n = 5) normalized to the expression in WT mice. *P < 0.05, one‐way ANOVA followed by LSD post hoc comparisons on qPCR data. (C) Effect of LPS exposure on the survival of WT and CypD^−/− mice (n = 12, labeling in the Figure).