Fig. 5.

CypD deficiency mitigates the suppressive effect of LPS exposure on Nrf2 regulated antioxidant defense. (A) IPA interaction analysis on the role of Myc and Atf4 in the regulation of Nrf2 target genes at 24 h after LPS exposure in WT mice. Red signs show upregulated genes while green signs represent downregulated genes. (B) Effect of CypD disruption on the LPS‐induced differential expression of Nrf2 target genes involved in glutathione‐mediated antioxidant defense. Red bars show significantly upregulated genes, green bars represent downregulated genes and gray bars show genes not significantly affected. ∞ denotes infinite FC value. Kal’s Z‐test, adjPval < 0.05, [FC] > 1.5. Please notice, genes with high FC values are presented on a different scale (n = 5). (C) qPCR validation of Nfe2l2, Sod2, Cat, Atf4, Myc, Gpx1, and Gstp1 mRNAs levels in sequencing data during LPS‐induced endotoxemia in WT and CypD^−/− mice liver. Data are presented as mean ± SEM of FC values normalized to the expression in WT mice, (n = 5). *P < 0.05, one‐way ANOVA followed by LSD post hoc comparisons on qPCR data.