Fig. 3.

MRGBP inhibits resection. (A, B) DNA‐end resection was scored by the accumulation of RPA foci 1 h after irradiation (10 Gy) in U2OS cells transfected with the indicated siRNAs. The average and SD of four independent experiments are plotted (A) and representative images are shown (B). Statistical significance was calculated using a one‐way ANOVA test comparing each condition to siControl cells. Scale bars represent 10 µm. (C–E) Resection length on individual DNA fibers was calculated using SMART. At least 200 fibers were scored per condition. Resection length was normalized to the control sample. The average and SD of four independent experiments are plotted (C). The quantification of a representative experiment is shown in D and representative images are shown (E). Each point corresponds to the length of a single DNA fiber. The median is shown in red.