Fig. 2.

(A) Early and late apoptotic events in bEnd.3 cells injured by H₂O₂ and the effects of various concentrations of NMN on 800 µm H₂O₂‐induced apoptosis were detected by flow cytometry. The top left quadrant indicates nonapoptotic cells; the top right quadrant represents late apoptotic events; the bottom right quadrant represents early apoptotic cells; and the bottom left quadrant represents living cells. B. The apoptosis rate of bEnd.3 cells induced by H₂O₂ that were pretreated with different concentrations of NMN was analysed by flowjo software. Results are expressed as means ± SD and are representative of three independent experiments. Data were analysed by one‐way ANOVA with LSD’s test for multiple comparisons. *P < 0.05, #P < 0.05 vs. the control group. #P < 0.05 vs. the 800 µm H₂O₂ group.