Fig. 3.

(A) Apoptosis of cells that induced by H₂O₂ (800 µm) and NMN (300 µm) reduced apoptosis induced by H₂O₂ was stained with Hoechst 33258. (B) The apoptotic and total cells were analysed by imagej software, and the apoptosis rate was presented as % of the total number of nuclei C. bEnd.3 cells were cultured with 800 µm H₂O₂ and 300 µm NMN (×10 scale bar = 100 μm). Results are expressed as means ± SD and are representative of three independent experiments. Data were analysed by one‐way ANOVA with LSD’s test for multiple comparisons. *P < 0.05, #P < 0.05 vs. control group. #P < 0.05, vs. 800 uM H₂O₂ group.