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. 2020 Dec 16;8:566932. doi: 10.3389/fcell.2020.566932

FIGURE 5.

FIGURE 5

miR-5001-5p targets the 3′-UTR of HES6 mRNA. (A) A Matrigel invasion assay was performed in SW1116 cells after transfection of control vector or CCMAlnc overexpression vector combined with HES6 siRNA. The invading cell numbers on each filter were counted. Data were plotted by defining the percentage of control cells as 100%. N = 3. Scale bars, 50 μm. (B) One of the predicted binding sites of miR-5001-5p with the 3′-UTR of HES6 mRNA. (C) Luciferase reporters containing HES6-3′-UTR or HES6-3′-UTR-mutant were cotransfected into DLD-1 cells with miR-5001-5p mimics or miR-5001-5p inhibitors. Luciferase activities were detected after 48 h. (D) Luciferase reporters containing HES6-3′-UTR or HES6-3′-UTR-mutant were cotransfected into SW1116 cells with miR-5001-5p inhibitors. Luciferase activities were detected after 48 h. (E) Luciferase reporters containing HES6-3′-UTR or HES6-3′-UTR-mutant were cotransfected into DLD-1 cells with CCMAlnc siRNA. Luciferase activities were detected after 48 h. (F) Luciferase reporters containing HES6-3′-UTR or HES6-3′-UTR-mutant were cotransfected into SW1116 cells with CCMAlnc overexpression vector. Luciferase activities were detected after 48 h.