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. 2021 Mar 4;19:39. doi: 10.1186/s12958-021-00723-2

Fig. 3.

Fig. 3

Interaction between Hsp90 and Erk1/2 during human sperm capacitation. a. Hsp90 was detected in sperm lysates using western blotting and Co-IP with non-immune mouse IgG or antibodies against Erk1/2. No interaction was observed between non-immune mouse IgG and Hsp90, whereas a clear interaction was evident between Hsp90 and Erk1/2. 17-AAG significantly disrupted the interaction between Hsp90 and Erk1/2. b. The relative association of Hsp90 and Erk1/2 with or without 5 µM 17-AAG during human sperm capacitation