Fig. 7.

MiR-580-3p targeted WEE1 to promote autophagy and suppress proliferation of glioma cells. Note: After transfected with miR-580-3p mimic or miR-580-3p inhibitor or pcDNA3.1-WEE1 or their negative controls, the expression of WEE1 was analyzed by qRT-PCR (a) and Western blotting (b). The number of acidic autophagosomes was examined through MDC (c) staining. Western blot assay was used to detect the expression of LC3-II/LC3-I, Beclin1 and p62 (d). Proliferation of U251 and SWO-38 was evaluated by CCK8 (e) and clone formation assay (f). FCM assessed cell cycle (g). * P < 0.05, ** P < 0.01, compared to mimic NC group. # P < 0.05, ## P < 0.01, compared to inhibitor NC group. & P < 0.05, && P < 0.01, compared to miR-580-3p mimic group. MDC, monodansylcadaverin staining; FCM, flow cytometry