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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Invest Dermatol. 2019 Dec 11;140(6):1266–1275.e3. doi: 10.1016/j.jid.2019.10.016

Figure 2. Significant decrease of TET-mediated 5-hmC in IMQ-induced mouse psoriasis.

Figure 2.

(a) Compared to untreated epidermis, psoriatic epidermis exhibits decreased 5-hmC. Nestin is upregulated in psoriatic lesions (S = sebaceous lobule). CK15-positive cells (encircled) express low 5-hmC but are undetectable in psoriatic epidermis (insets show CK15 and 5-hmC in hair follicle bulge region). FABP5 detected in sebaceous lobules (arrows) but not in control epidermis, whereas expression is present in psoriatic suprabasal keratinocyte cytoplasm and nuclei. Scale bar = 500 μm for HE. Scale bar = 100 μm for IHC. Downregulation of TET1 and TET2 (b) as well as upregulation of nestin (b) and IL-17A (c) gene expression is detected by RT-PCR in the IMQ-treated epidermis. ** P<0.01, ***P<0.001. Data shows mean ± SEM of n=3.