Skip to main content
. 2021 Feb 17;6(8):5786–5794. doi: 10.1021/acsomega.0c06191

Figure 3.

Figure 3

(a) Effects of compounds 1, 10, 10b, and 10c (indicated on the top) used at a concentration of 1 mM on the ability of CHIKV nsP2 to cleave a recombinant protein substrate. Names of the proteins are indicated on the right, and molecular masses of marker bands are indicated on the left. (b) Western blot analysis. BHK-21 cells infected with CHIKV-NanoLuc (MOI 10) were treated with increasing concentrations of compound 10. Cell lysates were collected 6 h post infection and run on 10% SDS-PAGE, and proteins were transferred onto the PVDF membrane. CHIKV proteins were detected using the respective rabbit primary antibodies and secondary anti-rabbit IRDye680-conjugated fluorescent antibodies. Loading control—β-actin—was detected using the primary mouse and secondary anti-mouse IRDye800-conjugated antibody. Names of the proteins are indicated on the left, and molecular masses of marker bands are indicated on the right. Neg: BHK-21 cells treated with 1% DMSO; Pos: infected BHK-21 cells treated with 1% DMSO (no inhibitor).