Table 3.
PCR results in cycle threshold (Ct) values of tissue samples from goats inoculated with a Vietnamese goatpox virus isolate (n = 8) and controls (n = 2)
| Goat No. | Group 1a | Group 2a | Group 3a | Control | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 335 | 129 | 373 | 269 | 287 | 261 | 265 | 752 | 759 | 267 | |
| Tissue | Ct value | |||||||||
| Subcutaneous tissue at injection site | 19.4 | 18.2 | 18.9 | 20.2 | 20.5 | NS | NS | NS | NS | NS |
| Skin crusts | 16.6 | 16.6 | 16.5 | 18.3 | 14.9 | 14.6 | 17.1 | 17.3 | NS | NS |
| Secondary nodules | NS | NS | NS | 20.8 | 22.1 | 22.6 | 24.7 | 31.4 | NS | NS |
| Testis | NS | 19.1 | NS | 31.4 | NS | NS | NS | NS | NS | NS |
| Trachea | 22.9 | 25.6 | NS | 23.5 | 32.8 | 30.8 | 36.4 | 36.3 | – | – |
| Lung | – | – | – | 36.2 | – | – | 35.7 | – | – | – |
| Pulmonary lymph node | 29.9 | – | 36.2 | 33.7 | – | – | 31.8 | – | – | – |
| Heart | – | – | 34.9 | 34.1 | NS | NS | NS | NS | – | – |
| Spleen | – | – | NS | 32.1 | 34.4 | – | – | – | – | – |
| Liver | – | – | NS | – | – | – | 35.1 | – | – | – |
| Rumen | NS | NS | NS | 29.8 | NS | NS | NS | NS | NS | NS |
| Abomasum | 37.2 | – | NS | 35.1 | NS | NS | NS | NS | NS | NS |
| Reticulum | 30.8 | NS | NS | 29.3 | NS | NS | NS | NS | NS | NS |
| Omasum | 24.3 | 35.9 | NS | 29.8 | NS | NS | NS | NS | NS | NS |
| Duodenum | – | – | NS | 33.9 | – | NS | NS | NS | NS | NS |
| Mesenteric lymph node | 34.4 | – | NS | NS | 34.1 | 36.5 | 36.7 | 34.7 | – | – |
| Kidney | 34.7 | – | NS | – | – | – | – | – | – | – |
| Blood | – | – | – | – | – | – | – | – | – | – |
Tissues were sampled at day 16 (group 1) post infection (pi) or day 18 pi (group 2, group 3, Control)
NS not sampled
–: Negative, i.e. below detection level
aGroup 1, Group 2, Group 3: Inoculated goats were retrospectively divided into 3 groups, based on severity of clinical signs at the end of the experiment