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. 2021 Mar;191(3):425–437. doi: 10.1016/j.ajpath.2020.09.004

Figure 1.

Figure 1

Fundoscopic evaluation of chronic uveitis disease activity. A: Following immunization in B10.RIII mice, digital fundus images were taken on a weekly basis, and graded on a scale of 0 to 4 by averaging the scores of optic disc, retinal vessel, and retinal tissue changes. Representative digital fundus images at indicated time points are shown. At week 2, EAU reaches disease peak, showing severe optic disc inflammation with some vessels in the optic disc masked (asterisk, scoring of 4/4), severe perivascular cuffing (yellow arrows, scoring of 4/4), and multiple retinal linear lesions (blue arrows, scoring of 4/4), which is finally graded as 4. In contrast, CAU at week 2 only shows slightly blurred optic disc margins (asterisk, scoring of 1/4) without vessel or retinal tissue changes (scoring of 0/4 for each), which is finally graded as 0.3. By week 10, EAU shows almost complete disease resolution with only isolated small retinal lesions identified (blue arrow, scoring of 1/4), which is finally graded as 0.3. However, CAU shows disease progression and reaches disease peak by week 12, exhibiting extensive inflammation in the optic disc and juxtapapillary area with some vessels in the optic disc masked and invisible (asterisk, scoring of 3/4), sclerotic vessels with moderate perivascular cuffing (yellow arrows, scoring of 3/4), and pan-retinal atrophy with many large lesions (blue arrows, scoring of 4/4), which is finally graded as 3.3. B: Following immunization in C57BL/6 (B6) mice, digital fundus images were similarly taken and scored. Representative digital fundus images at indicated time points are shown. At week 3, EAU reaches disease peak, showing swelling of optic disc and blurred optic disc margins (asterisk, scoring of 2/4), extensive perivascular cuffing (yellow arrows, scoring of 3/4), and diffuse and linear whitish lesions (blue arrows, scoring of 4/4), which is finally graded as 3. In contrast, CAU shows slightly blurred optic disc margins (asterisk, scoring of 1/4) with normally appearing retinal vessels and tissues (scoring of 0/4 for each), which is finally graded as 0.3 on average. By week 10, EAU shows complete disease resolution; yet, CAU shows disease progression and reaches disease peak by week 12 with slightly blurred optic disc margins (asterisk, scoring of 1/4), engorged vessels (yellow arrows, scoring of 1/4), and pan-retinal atrophy with numerous small lesions (blue arrows, scoring of 3/4), which is finally graded as 1.7. Data are presented as means ± SEM of a single representative experiment (out of two performed) of 14 eyes from seven mice in CAU group, and 10 eyes from five mice in each of EAU and control (mice receiving CFA and Bordetella pertussis toxin without the peptide) group (A and B). B6, C57BL/6.