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. 2021 Mar;191(3):425–437. doi: 10.1016/j.ajpath.2020.09.004

Figure 5.

Figure 5

Memory T-helper 17 (Th17) response in retinal tissues in C57BL/6 mice with chronic autoimmune uveitis (CAU). Retinal tissues were harvested and prepared for flow cytometric assessment at week 12. Frequencies and numbers of total CD4+ T cells in retina were determined after debris and doublet exclusions (A), and are summarized in bar chart (B). Furthermore, frequencies of IL17+IFN-γ-CD4+ (total Th17) and CD44hiIL-17+CD4+ (memory Th17) cells among total CD4+ T cells were examined with representative flow cytometry dot plots (C and E), and bar charts summarizing total and memory Th17 cells (D). CD44hi Th17 cells were further characterized according to the expression of the memory markers IL-7R and IL-15R (F). Results are from a single experiment (with each sample pooled from four eyes) and are representative of two performed. n = 3 per group. ∗P < 0.05 versus control. FSC, forward scatter; IFN-γ, interferon-γ.