Skip to main content
. 2021 Mar;191(3):425–437. doi: 10.1016/j.ajpath.2020.09.004

Figure 6.

Figure 6

Memory T-helper 17 (Th17) response in lymphoid tissues in B10.RIII mice with chronic autoimmune uveitis. Cervical draining lymph node (CLN), distal inguinal LN (ILN), spleen, bone marrow, and peripheral blood samples were collected and prepared for flow cytometric assessment at week 12. A: Gating strategy and isotype controls for analyzing total and memory Th17 cells. B: Representative flow cytometry histograms show the expression of IL-7R and IL-15R on CD44hi Th17 cells. Results are from a single experiment and are representative of two performed. C: Frequencies of IL17+IFN-γ−CD4+ (total Th17, white bars) and CD44hiIL-17+CD4+ (memory Th17, gray bars) cells among total CD4+ T cells were examined with representative flow cytometry dot plots shown on the left, and bar charts summarizing total and memory Th17 cells shown on the far right. n = 3 to 4 per group (B). ∗P < 0.05 versus control. FSC, forward scatter; IFN-γ, interferon-γ.