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. 2021 Mar 2;8(3):e975. doi: 10.1212/NXI.0000000000000975

Figure 1. DTH-TLS Model Development Characterization.

Figure 1

Focal MS-like brain lesions with TLS-like structures were established in mice. Mice were perfused on days 1, 6, 12, 28, 60, and 100, after which brains were analyzed for microglia (Iba-1), astrocytes (glial fibrillary acidic protein), B cells (B220), T cells (CD3), and myelin (MBP) by immunohistochemistry. The DTH-TLS model features a gradually expanding area of activated microglia and astrocytes, an increasingly growing lymphocyte compartment with B- and T-cell compartmentalization, and increasingly worsening demyelination. Representative micrographs are shown of n = 4 per time point. Red scale bars represent 100 μm. TLS = tertiary lymphoid structures.