FIGURE 2.

Pomc mRNA is transiently elevated in both female and male mice undergoing ABA. Representative confocal images taken at 40× of Pomc mRNA detected with Alexa488‐labeled probe in the arcuate nucleus of the hypothalamus from male animals sacrificed after 48 hr (ai) or 72 hours (aii) in ABA. Summary data from females are shown in panel b: *p = 0.0500, #p = 0.0177, one‐way ANOVA. Summary data from males are shown in panel c: *p = 0.0110, one‐way ANOVA. Summary data are presented as mean ± SD. Additional details are found in the results section. 3 V = third ventricle. FR = food restricted. WHL = wheel. n numbers were as follows: female: FR only: n = 6, WHL only: n = 6, 24 hr ABA: n = 5, 48 hr ABA: n = 6, 72 hr ABA: n = 3, male: FR only: n = 4, WHL only: n = 5, 24 hr ABA: n = 5, 48 hr ABA: n = 5, 72 hr ABA: n = 6