Figure 1.

Effect of polyamine treatment on PTPN2 activity in HT29 cells. HT29 cells were treated for 30 minutes with (A) spermidine, (B) spermine, (C) putrescine, (D) N¹-acetylspermidine, (E) N¹-acetylspermine, and (F) N⁸-acetylspermidine at the indicated concentrations. Depicted are phosphatase activity levels of PTPN2-immunoprecipitates normalized to precipitated PTPN2 protein level and representative Western blot pictures of PTPN2 immunoprecipitates. Data represent means (n = 3) and standard deviations. Analysis of variance ANOVA followed by post hoc Tuckey; asterisks indicate significant differences between the treatment groups and control (*P < 0.05, **P < 0.01).