Figure 2.

Spermidine is the most potent activator of PTPN2 in HT29 and T84 cells. A, HT29 cells and (B) T84 cells were treated for 30 minutes with 10 µM of spermidine, spermine, putrescine, N¹-acetylspermidine, N¹-acetylspermine, or N⁸-acetylspermidine as indicated. Depicted is PTPN2 activity normalized to PTPN2 protein content and representative pictures of Western blot from PTPN2 precipitates. Data represent means (n = 3) and standard deviations. Analysis of variance ANOVA followed by post hoc Tuckey; asterisks indicate significant differences between the treatment groups and control (***P < 0.001).