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. 2020 Feb 7;26(7):1038–1049. doi: 10.1093/ibd/izaa013

Figure 7.

Figure 7.

Effect of spermidine treatment on mRNA expression of IFN-γ target genes in THP-1 cells and IBD patient-derived PBMC expressing the C-variant. THP-1 cells expressing the T (“WT”) or the C (“variant”) allele in SNP rs1893217 were treated for 24 hours with 100 ng/mL IFN-γ and/or 10 µM spermidine. The graphs show mRNA levels of (A) ICAM1, (B) NOD2, and (C) PTPN2. Data represent means and standard deviations (n = 5). PBMC from healthy controls (HC, black bars, homozygous for the T allele, n = 5), or from IBD patients homozygous for the T allele (TT, blue bars, n = 6) or heterozygous (CT, red bars, n = 5) for the PTPN2 SNP rs1893217 were treated for 24 hours with 100 ng/mL IFN-γ and/or 10 µM spermidine. The graphs show mRNA levels of (D) ICAM1, (E) NOD2 and (F) PTPN2. Data represent means and standard deviations. Analysis of variance ANOVA followed by post hoc Tuckey; asterisks indicate significant differences between the treatment groups (*P < 0.05, **P < 0.01).