Skip to main content
. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: J Immunol. 2020 Sep 30;205(10):2649–2666. doi: 10.4049/jimmunol.2000459

Figure 1. Zbtb20 modulates glycolytic and mitochondrial respiratory capacity in effector and memory CD8 T cells differentiated in vitro.

Figure 1.

Total splenocytes were harvested from WT OT-I mice and GZB-cre Zbtb20-fl/fl OT-I (KO) mice, then activated with SIINFEKL peptide for 48h without exogenous IL-2. Activated cells were further cultured with 100U/ml rhIL- 2 or 50ug/ml rmIL-15 for 7 days. Cultured cells were then analyzed using Seahorse XFe96 Analyzer. (A) Oxygen consumption profile showing mitochondrial respiration and (B) proton efflux rate profile showing glycolytic metabolism for IL-2 cultured cells. (C) Mitochondrial respiratory capacity of IL-2 cultured cells. (D) Glycolytic capacity of IL-2 cultured cells. (E) Mitochondrial and (F) glycolytic metabolic profiles for IL-15 cultured cells. (G) Mitochondrial respiratory capacity for IL-15 cultured cells. (H) Glycolytic capacity of IL-15 cultured cells. Each group consisted of at least four replicates and each experiment was repeated three times. Each point represents data from an individual mouse. Statistics were performed with Student’s unpaired t-test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Representative data from three experiments are shown.