Figure 11. Zbtb20 deletion enhances the recall response of memory CD8 T cells and increases protection against MC38 tumor.

(A-D) OT-I cells were adoptively transferred and infected as described in Figure 7. On day 29 or day 81 post infection, recipient mice were challenged with MHV-68-Ova retro-orbitally. Splenocytes were harvested after re-challenge for flow cytometric analysis. (A-B) Cell counts for transferred OT-I cells from the entire spleen of recipients challenged on (A) D28 or (B) D80 post-infection. Each point represents data from an individual mouse. Each group comprised at least four mice and each experiment was repeated three times. Statistics were performed with Student’s unpaired t-test. **P<0.01, ****P<0.0001. (C-D) On D80 post infection, splenocytes were harvested from recipient mice and OT-I cells were purified. 10⁶ memory OT-I cells were transferred into MC38-Ova tumor-bearing mice (D4 post subcutaneous inoculation of MC38-Ova) and tumor area was measured. (C) Plot showing tumor growth in individual animals. Arrow represents time of T cell injection. Numbers at bottom right indicate the proportion in each group that did not grow measurable tumor (baseline on graph). (D) Time to endpoint curve, with the endpoint defined as the tumor area exceeding 400 mm². N=9 for KO group, N=10 for WT and no T cell transfer groups. Data is representative of three replicate experiments. Statistics were performed with 2-way ANOVA (C) or a Mantel-Cox log rank test (D). **P<0.01.