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. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: J Immunol. 2020 Sep 30;205(10):2649–2666. doi: 10.4049/jimmunol.2000459

Figure 8. Zbtb20 deletion affects CD8 T cell differentiation to memory precursors and effector CD8 T cells.

Figure 8.

Samples from the experiment described in Fig. 6 were used to measure cytokine production and memory precursor or effector differentiation on day 28 or day 60 post-infection. (A) Cell counts for transferred OT-I cells from the spleen of each recipient. (B) Representative dot plot showing KLRG-1 and CD127 staining and the percentage of memory precursors (KLRG-1-CD127+) and terminal effector cells (KLRG-1+CD127-) and quantification. (C) Representative dot plot showing TNF-α and IFN-γ staining and quantitation. (D) Representative dot plot showing IL-2 and IFN-γ staining and quantitation. (E) Representative dot plot showing CXCR3 and CD8 staining and quantitation. (F) Representative dot plot showing CD27 and CD8 staining and quantitation. Each point represents data from an individual mouse. Each group consisted of at least four mice and each experiment was repeated three times. Numbers in dot plot quadrants show percentage of events in the relevant quadrant. Statistics were performed with Student’s unpaired t-test. *P<0.05, **P<0.01, ***P<0.001.