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Comparison of the multiplex gene expression measurement technologies.
| Features | Profiling | Targeted | |||
|---|---|---|---|---|---|
| Microarray | RNA-Seq | qRT-PCR | TempO-seq | nCounter® | |
| Sensitivity | Medium | Medium/ High |
High | High | High |
| Need amplification and/or library | Yes | Yes | Yes | No | No |
| High-throughput | No | No | No | Yes | Yes |
| Automate | No | No | No | Yes | Yes |
| Cost of reagents | High | High | Medium | Medium | Medium |
| Cost of labor | High | High | Medium | Medium | Low |
| Readout | Analog | Digital | Analog | Digital | Digital |
