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. 2021 Mar 4;16(3):e0247858. doi: 10.1371/journal.pone.0247858

Fig 4. Characterizing the effect of the V617F mutation in CD34+ hematopoietic stem cells.

Fig 4

(a, b) Immunophenotypic analysis of methylcellulose colonies cultured with or without EPO. All colonies were scraped, pooled and stained the following surface markers. Lymphoid: CD19 or CD56; Myeloid: CD33, or CD14 or CD41a; Erythroid: CD71 or GlyA. Data from n = 1 for all except GlyA samples. GlyA data from n = 3 independent biological replicates. Mean±SD shown. *:p<0.05 by paired t-test. (c) Immunoblot showing the elevated expression of B-globin in V617F edited cells grown in methylcellulose without EPO. No difference was observed in P-STAT5 expression across all samples. GAPDH served as a loading control.