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. 2021 Mar 4;16(3):e0248158. doi: 10.1371/journal.pone.0248158

Fig 4. Macrophages are essential for Th2-elicited sneezing responses.

Fig 4

(A) Experimental schema. WT mice were adoptively transferred with OVA-Th2 cells. After 5 weeks, mice were intranasally challenged with OVA. On day 3, 6 h after the third nasal challenge, mice were intraperitoneally injected with PBS or clodronate-containing liposomes. (B) Number of sneezes was counted for 10 min immediately after each intranasal challenge. (C, D) Immediately after the final challenge, the frequencies of OVA-Th2 cells (CD4+DO11.10TCR+ cells in CD45+ cells) (C) and eosinophils (CCR3+Siglec-F+ cells in CD45+ cells) (D) in nasal mucosa were examined by FACS. Pooled data from 2 independent experiments are shown by means±SEM (B) or means±S.D. (C and D), n = 8. ****P<0.0001, N.S. not significant.