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. Author manuscript; available in PMC: 2021 Mar 4.
Published in final edited form as: ACS Appl Bio Mater. 2020 Mar 16;3(5):2838–2849. doi: 10.1021/acsabm.0c00101

Figure 4.

Figure 4.

Nonviral delivery of CRISPR-Cas9 system for gene editing. Cas9 ribonuclease can be delivered in the forms of Cas9-expressing DNA, Cas9-expressing mRNA, and Cas9 protein.116 sgRNA can be delivered by expression from sgRNA-coding DNA or as independent oligonucleotides together with Cas9-expressing mRNA or Cas9 proteins. Adapted with permission from ref 116. Copyright (2017) American Chemical Society Publishing Group.