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. 2021 Mar 4;16(3):e0247640. doi: 10.1371/journal.pone.0247640

Fig 2. SARS-CoV-2 spike pseudotyped virus neutralization assay.

Fig 2

SARS-CoV-2 spike pseudotyped lentivirus virus encoding EGFP were tested against negative control pre-pandemic plasmas (N1, N2 and N3) and COVID-19 plasmas (US1 to US17 and SSA1 to SSA3) at 1:40 plasma dilution. At 72-hours post-infection, percentage of GFP positive cells were quantified with BD Accuri C6 Plus flow cytometer. The mean of triplicate wells was shown with error bars representing SEM. P-values were calculated via one-way ANOVA and “*” denotes p < 0.05 relative to negative control plasmas.