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. 2021 Feb 19;10:e63524. doi: 10.7554/eLife.63524

Figure 2. The overall structure of ABCA4 in the absence of ATP.

(A) The domain structure of ABCA4. The N- and C-terminal halves of the molecule are shown in blue and green. (B) Two orthogonal views of ABCA4 in ribbon presentation. Also shown is the electron microscopy density corresponding to residues 138–271 in ECD1. Ordered detergents, lipids, and N-linked glycans are shown as stick model. The position of the membrane is indicated by two gray lines.

Figure 2.

Figure 2—figure supplement 1. Cryo-electron microscopy reconstructions of the ATP-free, wild-type ABCA4.

Figure 2—figure supplement 1.

(A) Summary of the image processing procedure. (B) Representative 2D class averages from cryoSPARC (Punjani et al., 2017). (C) Density map colored by local resolution estimation using RELION 3 (Scheres, 2012; Zivanov et al., 2018). (D) Fourier shell correlation (FSC) plots. Black: half map 1 versus half map 2; blue: model versus summed map. (E) Angular distribution of the particles used in the final reconstruction. Each sphere indicates the number of particles from this angle, and the size of the spheres corresponds to the number of particles.
Figure 2—figure supplement 2. Local density of the ATP-free, wild-type ABCA4 reconstruction.

Figure 2—figure supplement 2.