Table 1. Summary of electron microscopy data and structure refinement statistics for wild-type ABCA4.
| Data collection | |
|---|---|
| Microscope | Titan Krios (FEI) |
| Voltage (kV) | 300 |
| Detector | K2 Summit (Gatan) |
| Pixel size (Å) | 1.03 |
| Defocus range (μm) | 0.8–2.5 |
| Movies | 5226 |
| Frames/movie | 50 |
| DDose rate (electrons/pixel/s) | 8 |
| Total dose (electrons/Å2) | 75 |
| Number of particles | 1,375,284 |
| Model composition | |
| Non-hydrogen atoms | 15,789 |
| Protein residues | 1941 |
| Lipids Digitonin |
14 1 |
| Sugar molecules | 14 |
| Refinement | |
| Resolution (Å) | 3.3 |
| Sharpening B-factor (Å2) | −99.8 |
| Root-mean-square deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 1.185 |
| Validation | |
| Molprobity score | 2.16 |
| Clashscore, all atoms | 18.86 |
| Favored rotamers (%) | 97.10 |
| Ramachandran plot (%) | |
| Favored | 94.28 |
| Allowed | 5.72 |
| Outliers | 0.0 |