Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (SARS-CoV-2) | pTT5 expression vector | GenBank | QHD43416.1 | For recombinant S protein |
| Gene (ACE2) | pHL-sec expression vector | GenBank | AB046569.1 | For recombinant ACE2 protein |
| Cell line (Homo sapiens) | Human embryonic kidney (HEK293-6E) | NRC, Canada | RRID:CVCL_HF20 | |
| Cell line (Homo sapiens) | Expi293F | Thermo Fisher Scientific | RRID:CVCL_D615 | |
| Antibody | Anti-human IgG Fc HRHorseradish Peroxidase (HRP)(goat polyclonal) | Thermo Scientific | RRID:AB_2536544 | WB (1:5000) |
| Recombinant DNA reagent | pHLmMBP-10 (plasmid) | Addgene, USA | RRID:Addgene_72348 | For recombinant RBD protein |
| Recombinant DNA reagent | pTT5 expression vector (plasmid) | Addgene, USA | RRID:Addgene_52367 | |
| Recombinant DNA reagent | pHL-sec expression vector (plasmid) | Addgene, USA | RRID:Addgene_99845 | recombinant DNA reagent |
| Chemical compound, drug | 3,3′,5,5′-Tetramethylbenzidine | Sigma Aldrich | RRID:AB_2336758 | |
| Chemical compound, drug | Deuterium oxide (chemical) | Cambridge Isotope Laboratories | CAS# 7789-20-0 | Deuterium exchange experiments |
| Software, algorithm | DynamX | Waters Corporation (Milford, MA) | Version 3.0 | |
| Software, algorithm | ProteinLynx Global Server (PLGS) | Waters Corporation (Milford, MA) | Version 3.0.1 | |
| Software, algorithm | GraphPad Prism software | GraphPad Prism (https://graphpad.com) | RRID:SCR_002798 | Version 5.0.0 |
| Software, algorithm | Modeller | 1989–2020 Andrej Sali | RRID:SCR_008395 | Version 9.21 |
| Software, algorithm | Visual molecular dynamics | University of Illinois at Urbana-Champaign | RRID:SCR_001820 | Version 1.9.3 |