Fig. 1.

Aseptic surgery setup inside a laminar flow hood. (a) The oxygen aids delivery of vaporized isoflurane to both the anesthesia chamber and the nose cone via Y-connection. The charcoal filter collects the waste anesthetic gas. A nitrile glove is cut and secured around the nose cone using a rubber band. Then the nose cone is secured to the surgery board using duct tape. (b) Surgery tools are autoclaved prior to use and maintained as sterile when placed in the laminar flow hood by using the packaging from the sterile surgical gloves. Surgery tools are also sterilized in a hot bead sterilizer between animals