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Evaluation of EVs isolated from B16BL6 cell media using dcTFF. (a) Schematic of dcTFF showing operation of the circulation system process. (b)–(e) DLS measurements of size distribution and TEM images of EVs at each size cutoff from B16BL6 cell culture media prior to filtration (b), from sample chamber C1 (c), from isolation chamber C2 (d), and from waste chamber C3 (e).
