Table 2.
Cell culture and RT-qPCR from original tissue samples and swabs; SARS-CoV-2 RNA was determined in the lungs of all four cases and further in the tissue sample of the brain in case 3 and in samples of the small intestine and kidney of case 2. Most samples of the respiratory tract in cases 2 and 4 showed CPE in cell culture as a sign of infectivity. CPE cytopathogenic effect, Ct cycle threshold. Not tested: if there was no original tissue or swab supernatant sample left, no RT-qPCR was performed
| Samples | Case 1 | Case 2 | Case 3 | Case 4 | |||||
|---|---|---|---|---|---|---|---|---|---|
| CPE | PCR (Ct) | CPE | PCR (Ct) | CPE | PCR (Ct) | CPE | PCR (Ct) | ||
| Swabs | Perioral | No | Not tested | No | Not tested | No | Not tested | Yes | Not tested |
| Right elbow (inner part) | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Left elbow (inner part) | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Right palm | No | 35.55 | No | Not detected | No | Not detected | No | Not detected | |
| Left palm | No | 31.87 | No | Not detected | No | Not detected | No | Not detected | |
| Oropharynx | No | Not tested | Yes | Not tested | No | Not tested | No | Not tested | |
| Trachea | No | Not tested | Yes | Not tested | No | Not tested | Yes | Not tested | |
| Right lung | No | Not tested | Yes | Not tested | No | Not tested | Yes | Not tested | |
| Left lung | No | Not tested | Yes | Not tested | No | Not tested | Yes | Not tested | |
| Tissue | Brain | No | Not detected | No | Not detected | No | 36.94 | No | Not detected |
| Thyroid gland | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Right lung | No | 30.72 | Yes | 24.81 | No | 34.46 | Yes | 25.08 | |
| Left lung | No | 30.51 | Yes | 14.96 | No | 34.40 | Yes | 20.88 | |
| Heart | No | Not tested | No | Not tested | No | Not detected | No | Not tested | |
| Liver | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Spleen | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Pancreas | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |
| Left kidney | No | Not tested | No | 34.83 | No | Not detected | No | Not tested | |
| Small intestine | No | Not detected | No | 20.99 | No | Not detected | No | Not detected | |
| Colon | No | Not detected | No | Not detected | No | Not detected | No | Not detected | |