Figure 3.

Cd81 expression levels correlated with the maturation state of β-cells. (A) Heat map representing the gene expression of the top 30 genes differentially regulated in P16 Cd81^high and Cd81^low/- β-cells. (B) Pathway enrichment analysis obtained from processing the differentially regulated genes of P16 Cd81^high and Cd81^low/- β-cells. (C) Violin plots of pivotal genes playing a crucial role during the maturation process of β-cells. Ucn3, Slc2a2 (Glut2), and Mafa are upregulated in Cd81^low/- β-cells and Mafb and Cgha are upregulated in Cd81^high β-cells. (D) Venn diagrams representing the comparison between differentially expressed genes of P16 Cd81^high and Cd81^low/- β-cells with highly upregulated genes from embryonic Fev⁺ (left) and endocrine cells (right) described in Bastidas-Ponce et al., 2019. (E) Venn diagrams representing the comparison between differentially expressed genes of P16 Cd81^high and Cd81^low/- β-cells with highly upregulated genes from β1 (left) and β2 (right) β-cells as described in Sachs et al., 2020. (F) Targeted analysis of specific signaling pathways differentially regulated between P16 Cd81^high and Cd81^low/- β-cells.