Figure 6.

CD81 expression patterns in healthy and stressed human β-cells. (A) Violin plots indicating CD81 expression levels in different pancreatic cells during in vitro differentiation of endocrine cells from iPSCs. (B) Immunostaining of CD81 expression in stage (S) 6 of human endocrine cells derived from iPSCs. Scale bar 20 μm. (C) Bar plot showing the percentage of CD81^high cells in different endocrine cell types using scRNA-seq of healthy adult human islets. (D) GO-terms enrichment analysis for differentially regulated genes of human CD81^high and CD81^low/- β-cells. (E) Targeted analysis of specific signaling pathways related to ER stress coping mechanisms differentially regulated between human CD81^high and CD81^low/- β-cells. (F) Venn diagram showing a comparison between differentially expressed genes of human CD81^high and CD81^low/- β-cells with β-cell clusters described in Xin et al., 2018. (G) Immunostaining for CD81 (green) in control and treated EndoC-βH1 cells. Scale bar 40 μm. (H) Quantification of the intensity levels of CD81 staining in EndoC-βH1 cells (2 independent experiments; ∗P < 0.05; ∗∗P < 0.01; t-test).