FIGURE 1.

Evaluation of the soluble expression of 196 genes of interest (GOIs) in Escherichia coli and Vibrio natriegens. (A) Flowchart about shake-flask fermentation of 196 GOIs and soluble expression comparison between E. coli BL21(DE3) and V. natriegens VnDX. (B) Compare the soluble expression of the same GOI in E. coli and V. natriegens through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Here, 0.15 OD₆₀₀ (10-well gel) or 0.075 OD₆₀₀ (15-well gel) prepared crude protein samples were loaded to the corresponding protein gel wells. V, V. natriegens VnDX; E, E. coli BL21(DE3); Blue box, GOI possessing higher expression in VnDX than BL21(DE3); Orange box, GOI possessing the same expression in VnDX and BL21(DE3); Gray box, GOI possessing higher expression in BL21(DE3) than VnDX. 1#: Alanine racemase from Bacillus subtilis 168; 2#: N-acetyl amino acid racemase from Alcaligenes sp.; 3#: N-acetyl amino acid racemase from Deinococcus radiodurans NCHU1003; 4#: N-acetyl amino acid racemase from Amycolatopsis sp. TS-1-60; 5#: Maltooligosaccharide trehalose synthetase from Arthrobacter sp. Q36; 6#: Maltooligosaccharide trehalose synthetase from Arthrobacterium S34; 7#: D-carbamoylase (K34E) from Burkholderia pickettii. (C) Pie chart about the soluble expression results of 196 GOIs. VnDX > BL21(DE3): GOI possessing higher expression in VnDX than BL21(DE3); VnDX = BL21(DE3): GOI possessing the same expression in VnDX and BL21(DE3); VnDX < BL21(DE3): GOI possessing higher expression in BL21(DE3) than VnDX.