Inhibition prior to tumor cell injection results in reduced tumor burden as well as ascites. (A) Schema of prophylactic dosing (B) Similar decease in ascites volume is seen with anti-TGF-β antibody administered prior to tumor challenge compared to treatment over five weeks. (C) Reduction in omental weights with single dose of anti-TGF-β antibody prior to tumor cell injection. (D-F) Again, favorable changes in T-cell response (D) increased proportion of CD8 T cells, (E) decrease in Tregs and (F) increased in CD8:Treg ratio, occur with this treatment strategy. (* p < 0.05, ** p < 0.01, bars represent SEM; dotted line – mean weight of naïve mouse omentum).