KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Viral Strains | ||
| AAV1.Syn.Flex.GcaMP6s.WPRE.SV40 | Addgene | 100845 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dulbecco’s phosphate-buffered saline | HyClone | SH30013.04 |
| Paraformaldehyde | MP Biomedicals | 150146 |
| Isoflurane | Clipper | 0010250 |
| Meloxicam | Norbrook Laboratories | 55529-040-11 |
| Bupivacaine | Moore Medical | 52683 |
| Hydrogen peroxide | Ricca Chemical Company | R3821310-1BV |
| Sodium chloride (NaCl) | Sigma-Aldrich | S7653-250G |
| Sterile saline | Pfizer | 00409-4888-12 |
| Intralipid® | Fresenius Kabi | NDC 0338-0519-13 |
| D-glucose | Sigma-Aldrich | G8270-100G |
| Ensure Plus, Vanilla | Abbott | 53642 |
| Proteinex- 18 Liquid solution | Llorens Pharmaceutical | 54859-535-30 |
| Cholecystokinin Octapeptide | Bachem | 4033010 |
| Methyl α-D-glucopyranoside | Sigma-Aldrich | M9376 |
| D-Mannitol | Sigma-Aldrich | M4125 |
| Phlorizin | Tocris | 4627 |
| Phloretin | Sigma-Aldrich | P7912 |
| Orlistat | Sigma-Aldrich | O4139 |
| Oleic Acid | VWR | 112-80-1 |
| Trisodium citrate dihydrate | Sigma-Aldrich | S1804 |
| Heparin sodium | Midwest Vet | 191.46750.3 |
| Glycerol | Sigma-Aldrich | G7893 |
| Urea | Sigma-Aldrich | PHR1406 |
| Potassium Chloride | EMD Millipore | 529552 |
| 3-O-Methyl-D-glucopyranose | Sigma-Aldrich | M4879 |
| Cyanoacrylate glue | Loctite | 4061 |
| Premilene Mesh® | B. Braun | 1064435 |
| Experimental Models: Organisms/Strains | ||
| Mouse:AgRP-IRES-Cre Agrptm1(cre)Lowl/J | The Jackson Laboratory | 012899 |
| Mouse: C57BL/6J | The Jackson Laboratory | 000664 |
| Software and Algorithms | ||
| MATLAB R2016a | MathWorks | https://www.mathworks.com/product/matlab.html |
| Synapse | Tucker-Davis Technologies | http://www.tdt.com/Synapse/index.html |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Microliter syringe pump, PHD Ultra | Harvard Apparatus | 703007 |
| Optic fibers for fiber photometry | Doric | MF2.5, 400/430-0.48 |
| 405 nm LED | ThorLabs | M405F1 |
| 490 nm LED | ThorLabs | M470F3 |
| Amplifier | Tucker-Davis Technology | RZ5P |
| Femtowatt photoreceiver | Newport | 2151 |
| Metabond | Parkell | S380 |
| Ortho-jet BCA Liquid | Lang Dental Manufacturing | B1306 |
| Jet Tooth Shade Powder | Lang Dental Manufacturing | 143069 |
| Micro-Renathane® Tubing | Braintree Scientific, Inc. | MRE025 |
| Micro-Renathane® Tubing | Braintree Scientific, Inc. | MRE033 |
| One Touch Ultra2 System Kit 1 | One Touch | |
| One Touch Ultra Blue Mail Order Test Strips, 50 CT | One Touch | 020-963 |
| Mouse/Rat Noradrenaline ELISA | Eagle Biosciences | NOU39-K01 0 |