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. Author manuscript; available in PMC: 2021 Jul 25.
Published in final edited form as: Nat Neurosci. 2021 Jan 25;24(3):379–390. doi: 10.1038/s41593-020-00772-7

Extended Data Fig. 9. Histological verification, ICSS and RTPP behavioral controls for vArky optogenetic stimulation.

Extended Data Fig. 9

(a) Infection site in VP and terminal fields in NAcSh; scale bar=50μM. (b) Verified placements of optic fibers. (c) Distance traveled in an open field task (n=12 eYFP, 18 ChR2, eYFPNoStim: 121.62±22.63, eYFPStim : 122.15±21.75, p=0.96, ChR2NoStim: 124.48±16.22, ChR2Stim: 121.83±14.18, Fstim•virus=0.050, p=0.825). (d) Preference for the stimulation-paired side of a chamber in a real-time place preference task (n=12 eYFP, 18 ChR2, eYFP: −4.36±4.32%, ChR2: −1.13±5.83) and representative heatmaps. *p<0.05, **p<0.01. All data presented as mean ± sem.