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. 2021 Mar 4;11:5154. doi: 10.1038/s41598-021-84647-2

Figure 3.

Figure 3

USP7 knock-down in T-ALL cell lines inhibits E-protein targets. Short hairpin RNAs (shRNA) targeting exon 4, 8, and 21 used for knock-down experiments (A). Western blot showing the reduction of USP7 protein after shRNA knock-down with the bar plot showing the actin normalized USP7 expression in Jurkat (B) and HSB-2 (C). GSEA analysis showing shRNA knock-down reduces the expression of E-protein targets compared to the controls in Jurkat (D) and HSB-2 (E). The number of RNA-seq samples used for this analysis is shown in Supplementary Table S10.