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. 2021 Mar 4;12(3):242. doi: 10.1038/s41419-021-03531-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Representative image of AgNOR stained human breast cancer tissue sections classified as TNBC or non-TNBC. Red arrows point at nucleoli. B Quantification of nucleolar number (using AgNOR staining) in human breast cancer tumor tissue sections classified as TNBC or Non-TNBC. Number of nucleoli per nucleus from cells in each specimen were counted. The data is represented as a box and whiskers plot. C Cells from each human breast cancer tumor tissue section, classified as TNBC or non-TNBC were separated into groups of cells with one nucleolus/nucleus, two nucleoli/nucleus, and three or more nucleoli/nucleus. These groups were represented as percent cells in each group. D Representative images of NucleolarID^TM stained cells from TNBC or Non-TNBC cell lines. Focused bright green staining demarcates nucleoli. DAPI staining (blue) marks the nucleus. E Quantification of nucleolar number (using NucleolarID^TM staining) in human breast cancer cell lines. The number of nucleoli per nucleus from six TNBC and six non-TNBC cell lines was counted. The data is represented as a box and whiskers plot and was significantly decreased in the non-TNBC cell lines (P < 0.001). Significance was determined using a T-test and all error bars indicate SEM.