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. 2021 Mar 4;11:5267. doi: 10.1038/s41598-021-84443-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of α-syn expression suppresses cell proliferation in vitro. (a) BrdU cell proliferation assay for control, KO and KI clones. Cells were probed with anti-BrdU antibody followed by FITC-conjugated secondary antibody (green) and counter staining the nuclei with DAPI (blue). Scale bar 50 μm magnification ×20 (n = 3). (b) Quantitative data expressing BrdU positive cells (in green) was evaluated by counting the positively stained cells in a blinded fashion by an unbiased observer utilizing Image J software. The average number of BrdU positive cells across 3 fields/slide was used to denote the total number of positively stained cells and expressed as percentage of BrdU positive cells. A one-way ANOVA with Dunnett test was performed to calculate p values.