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. 2021 Mar 4;12:1441. doi: 10.1038/s41467-021-21691-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunostaining of CSR-1 and PIWI in 1-, 4-, 8-, and more than 100-cell embryos of a 3xFLAG::HA::CSR-1 CRISPR-Cas9 strain, using anti-FLAG and anti-PRG-1 antibodies. DAPI signal is shown in blue. Scale bars represent 10 µm. b Brood size assays of CSR-1 degron strains with and without Auxin. The data points correspond to the number of living larvae from individual worms. Data are presented as mean ± SD. Two-tailed P values were calculated using Mann–Whitney–Wilcoxon tests. The sample size n (worms) is indicated in parentheses. c Percentages of embryonic lethality from the brood size experiment shown in b, measured as the percentage of dead embryos versus the total number among laid embryos. Data are presented as mean ± SD. The sample size n (worms) is indicated in parentheses. d Percentage of embryonic lethality in CSR-1 degron strains complemented with single-copy transgenic expression of CSR-1 with (ADH) or without (DDH) mutation in the catalytic domain. The percentage of embryonic lethality is calculated by dividing the number of dead embryos by the total number of laid embryos. Dots correspond to the percentages of embryonic lethality from individual worms. Data are presented as mean ± SD. Two-tailed P values were calculated using Mann–Whitney–Wilcoxon tests. The sample size n (worms) is indicated in parentheses. The graphical representation of the experiment is shown on the left. Source data are available online.