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. 2021 Mar 4;11:5130. doi: 10.1038/s41598-021-84384-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of HepG2 embedded in algMC with Matrigel – EdU proliferation assay. Cell nuclei were stained with DAPI (blue); representing original cells, while cells labelled with EdU are shown in green; representing newly formed DNA and therefore mitosis. Upper tile (20 × magnification) shows the distribution of newly formed cells allover a specific region of the scaffold. Lower tile (40 × magnification) shows distribution of cells within the formed clusters and their rate of proliferation; scale bars represent 100 µm.