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. 2021 Feb 19;12:627785. doi: 10.3389/fmicb.2021.627785

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Copyright © 2021 Wang, Han, Tian, Chen, Gao, Wu, Liu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological observation of the pathogenic strain M228 and its mutants infecting leaves of kiwi by SEM. (A) Healthy kiwi leaves; (B) after 4 days of inoculation of the host kiwifruit leaves using wild-type strain M228, a large number of pathogens could be observed in the host tissue. (C,E) After 4 days of inoculation of the host kiwifruit leaves using the mutants M228ΔtssM and M228ΔtssJ, only a small amount of bacterial colonization was found in the host tissue; (D,F) after 4 days of inoculation of the host kiwifruit leaves using the mutantsM228ΔtssM-R and M228ΔtssJ-R, a large number of pathogens could be observed in the host tissue, and there was no significant difference in the colonization and infection ability between the wild-type strain M228 and the complemented strains. UE, upper epidermis; PT, palisade tissue; ST, sponge tissue; PH, phloem; XY, xylem; LE, lower epidermis; LVB, lateral vein; B, pathogenic bacteria.