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. 2021 Feb 19;12:627785. doi: 10.3389/fmicb.2021.627785

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Copyright © 2021 Wang, Han, Tian, Chen, Gao, Wu, Liu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential expression of T3SS-related genes analysis in the process of pathogenic strain Psa M228 and its mutant M228ΔT6SS infecting kiwi branches. During the interaction between the mutant strain M228ΔT6SS and the host, the expression levels of the T3SS transcriptional regulatory genes hrpR, structural genes hrpZ, hrcC, hopP1, and effector genes hopM1 and hopH1were regulated. Following interaction for 2 h, T3SS-related genes hrpR, hrpZ, hrcC, hopP1, hopH1, and hopM1 of the mutant M228ΔT6SS, were down-regulated by 2.01, 1.48, 1.46, 1.14, 1.05, and 1.2 times compared to the wild-type strain; following interaction for 16 h, the expression of the genes was down-regulated by 2.71, 1.76, 1.70, 1.48, 1.78, and 1.83 times, using gyrB as internal reference; a similar downregulation result was obtained using dusA and ftrA as internal reference genes.