Figure 1.

Qualitative and quantitative assessment of the ability of RABV to infect mature human monocyte-derived macrophages. Human monocyte-derived macrophages (MDMs) were exposed to street RABV at a MOI of 0.1, 1, 10 or 50, and at 72 h post-infection (hpi) cells were fixed and stained with an antibody against the RABV-N protein (A). The white scalebar represents 500 μM and all images were acquired at the same magnification. In parallel, supernatants of 8, 24, 48, and 72 hpi (MOI 0.1 and 1; n = 6) or 72 hpi (MOI 10 and 50; n = 2) were titrated on MNA cells (B). Bars represent the mean ± SEM of two-six donors; for each condition, two wells were infected per condition and all supernatants were titrated in triplicate. Highly susceptible MNA and SK-N-SH cells were taken along as positive controls.