Skip to main content
. 2021 Feb 19;13:593927. doi: 10.3389/fnagi.2021.593927

FIGURE 2.

FIGURE 2

CM from adult 3xTg-AD astrocytes decreased viability and generated filopodia-like dendritic protrusions in neurons in vitro. (A) Immunofluorescence of neurons treated with CM from control or 3xTg-AD astrocytes. Hoechst nuclear staining is blue. Phalloidin-Alexa 488 staining of F-actin is green. MAP2-Alexa 594 staining of microtubule-associated protein 2 is red. Calibration bar: 20 μm. Details of condensed nuclei at 50% zoom, calibration bar: 5 μm. (B) Quantification of the average percentage of condensed nuclei. Average number of condensed nuclei was compared in various treatments. n = 3; ANOVA comparisons of 3xTg-AD with *Control; #CM-C57BL6; °CM-PS1-KI. P-value significance: ###0.001; **0.01; °0.05. (C) Neurons exposed to CM of astrocytes during 24 h; calibration bar: 20 μm. Details in the dotted rectangle show 100% magnification of the dendrites with the spines; calibration bar: 5 μm. (D) Distribution of the number of spines according to the length range. n = 3; ANOVA comparisons of 3xTg-AD with #CM-C57BL6. P-value significance: #0.05. Kruskal-Wallis analysis of non-normally distributed data. P-value significance: +0.05.