Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 4;11:5222. doi: 10.1038/s41598-021-84538-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The triple energetic blockade alters the energetic machinery. Oxidative phosphorylation was evaluated by measuring OCR (a) and ECAR (b) prior to and after injecting oligomycin, FCCP, and a combination of rotenone (R) and antimycin A (Aa). (c) Glycolysis was evaluated by measuring ECAR prior to and after injecting glucose, oligomycin, and 2-deoxy-D-glucose (2-DG). (d) Energetic phenotype diagrams involving OCR and ECAR, under basal measurement and under the maximal stress after the injection of FCCP. (e–f) Individual parameters for oxidative phosphorylation (e) and for glycolysis (f). (g-h) Cellular respiration corresponding to substrate capacity (g) and dependency (h). (i,j) Fuel oxidation diagram representing the flexibility and dependency towards the three energetic substrates with OLD control (i) or OLD (j). The sum of flexibility and dependency indicates fuel capacity. Each condition was compared against its control. Data are expressed as means ± s.e.m. N = 3 independent experiments. Statistical analyses were performed with two-tailed unpaired Student t-test with Holm-Sidak correction. OLD: Orlistat + lonidamine + DON; GII: Growth hormone + insulin + indomethacin; 6 drugs: OLD + GII; GlucC: Glucose capacity; LC: Long-chain fatty acid capacity; GlutC: Glutamine capacity; GlucD: Glucose dependency; LD: Long-chain fatty acid dependency; GlutD: Glutamine Dependency; *p < 0.05; **p < 0.01.